Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom

Research Report

J Venom Res (2011), Vol 2, 11-16

Published online: 25 May 2011

Full Text: (PDF ~541kb) | (PubMed Central Record HTML)

Mariane Assafim †, Eduardo Coriolano de Coriolano †‡, Sérgio Eufrázio Benedito †, Caio Pinho Fernandes ‡, Jonathas Felipe Revoredo Lobo ‡, Eladio Florez Sanchez §, Leandro Machado Rocha ‡, André Lopes Fuly †*

† Instituto de Biologia, Departamento de Biologia Celular e Molecular, Universidade Federal Fluminense, Niterói, RJ, Brazil

‡ Faculdade de Farmácia, Universidade Federal Fluminense, Niterói, RJ, Brazil

§ Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil

*Correspondence to: André Lopes Fuly, E mail: andfuly@vm.uff.br, Tel: +55 21 26292294, FAX: +55 21 2629 2376

Received: 15 March 2011, Revised: 23 May 2011, Accepted: 25 May 2011

© Copyright The Authors


Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract ofHypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubatingB. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararacavenom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararacaenvenomation.

KEYWORDS: Hypericum brasiliense , medicinal plant, Bothrops jararaca, snake venom, antivenom